Cytogenetics of subterranean Los Baños termite, Microcerotermes losbañonesis (oshima).

Date

10-2004

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Lacto-aceto orcein squash preparations of the eggs of subterranean Los Banos termite, Microcerotermes losbationensis (Oshima), sampled from UPLB Nursery were used to investigate the qualitative and the quantitative features of cells and chromosomes. Prophase cells are characterized by round, darkly staining, clumped chromatin on the nuclear region. Its typical mean length, width, and area are 7.71, 6.59, and 50.96 pm, respectively. Metaphase is distinguished by the alignment of thicker and shorter chromosomes at the equatorial plane, and the appearance of spindle fibers. Its cells are larger compared to Prophase cells as shown by its mean length, width, and area of 8.81, 7.12, and 66.56 pm, respectively. Anaphase showed the movement of chromosomes toward opposite poles. The cell's mean length, width, and area are 11.99, 8.45, and 101.99 pm, respectively. Finally, at Telophase, there was uncoiling of chromatids, reconstitution of nuclear envelope, and cytokinesis. Its mean length, width, and area are 12.88, 8.76, and 112.41 pm, respectively. Interpretative drawing of the chromosomes and karyogram constructed at Prometaphase revealed a chromosome number of 2n---42, and total relative chromosomal length range of 7.0 to 21.0 mm. Total number of dividing and non-dividing cells were 95 and 3,374, respectively. The mean mitotic index was 3.09.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

This document is currently not available here.

Share

COinS