Genetic variability in grain mineral density and molecular profiling of elite donor accessions of rice (Oryza sativa L.)

Date

4-2008

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn O. Villa

Abstract

Fifty-seven (57) accessions of rice (0ryzo sativa L.) were grown and evaluated for genetic variability in grain mineral density. Dehulled rice grains were analyzed using the Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-ES) which showed that four (4) accessions. namely IR 76569-166-4-2-2, IR 71524-44-1-1. IR 71 137-243-2- 2-3-3, and 1R75386-14-3-2-2 had the highest iron (17 mg/kg), zinc (34 mg/kg), phosphorus (0.287%), and calcium (0.022%) density or concentration, respectively. DNA fingerprinting was also done using 67 SSR primers on chromosomes 1-12 to evaluate the degree of polymorphism and genetic variability among the accessions. Polymorphism information content (PIC) for these rice microsatellite markers (RM) ranged from 0.0175 (RM 133) to 0.8441 (RM21). Four (4) dinucleotide markers had the highest PIC values (>0.70): RM154 (0.7003), RM413 (0.7429), RM204 (0.7853). and RM21 (0.8441). RM21 could be recognized as the most useful marker for characterizing the elite donor accessions, followed by RM204, RM413, and RM154. Cluster analysis of 30 representative elite accessions that were subjected to grain mineral concentration analysis revealed four (4) clusters. with one cluster containing the two accessions with the highest grain iron and zinc concentration. Cluster analysis of the 57 elite accessions revealed live (5) clusters. Irrigated accessions grouped into two clusters. This information obtained can be used for selection of parents in breeding for modern high-yielding varieties with high grain mineral content.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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