Morphological anatomical and cytological characterization of tomato (Solanum lycopersicum L.) lines introgressed with S. pennellii L.

Date

4-2009

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

The morphology, anatomy and cytology of tomato, S. lycopersicum L. lines introgressed with S. pennellii L. were studied. Morphological characterization was based ron the descriptors for Tomato (Lycopersicon spp.) provided by the International Plant Genetic Resources Institute (IPGRI). Seven introgression lines (ILs) were each associated with a unique qualitative trait, among which were IL 4-4 associated with indeterminate growth habit and IL 3-2 with yellow color of mature fruit. Based on quantitative traits, IL 5-2 is significantly different from the rest based on thickness of pericarp, IL 7-4 on number of locules and pedicel length and IL 7-4-1 based on thickness of fruit wall. Microtome sampling was performed for the leaf characterization where stomatal density and index were computed. Stomatal density was highest in IL 4-3-2 (462.60) and lowest in IL 8-1-1 (190.93). S. lycopersicum cv. M-82 (284.47) had a higher stomatal density than S. pennellii LA716 (253.40). The overall adjusted mean stomatal density of the ILs (311.34) was higher than both parental lines. Stomata] index was highest in ILs 7-3 and 7-4-1 (0.34) and lowest in ILs 2-2, 5-1, 5-5 and 8-1-1 (0.24). Stomatal index in M-82was 0.28 while LA716 and the ILs both had an average of 0.29. Using iron-acetocarmine squash technique, the chromosome behavior in meiosis I of the ILs was analyzed. Diakinesis showed a prevalence of 12 II for the four ILs observed. A 2 IV + 811 configuration was observed in IL 2-6-5(8%), 1 IV + 9 II + 2 I in IL 3-4 (8%), 1 IV + 10 II in all lines, and 11 II + 2 I in all lines except for IL 1-1-3. Laggards ranging from 12% to 22% during metaphase I were observed in all lines. Early disjunction of chromosomes was also observed ranging from 4% to 10%. Normal telophase stage was observed in all PMCs of IL 1-1-2. Laggards were present in 2% of ILs 1-1-3 and 2-6-5, and 6% in IL 3-4. Pollen fertility was estimated as the percentage of pollen grains that could be stained with acetocarmine solution. For the pollen fertility analysis, only 7 ILs had more than 50% fertile pollens. As compared to S. lycopersicum TL01519 with 92.67% pollen fertility, the highest pollen fertility among the ILs was observed in IL 7-4 (87.89%) while the rest of the ILs exhibited fairly low pollen fertilities ranging from 11% to 47.78%.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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