Meiosis in three Philippine Medinillia species (Melastomataceae)

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ma. Cecilia S. Reamillo

Co-adviser

Simeona V. Siar

Abstract

Chromosome number, meiotic behavior, and pollen fertility of Medinilla cumingii Naud., M. pendula Merr. and Medinilla sp. were studied using acetocarminc squash technique on Farmer's solution-fixed flower buds gathered between 9:00-11:00 am. Diakinesis showed 171Is in 56.45% of observed M. cumingii cells, and 211Is for both M. pendula (67.92%) and Medinilla sp. (56.76%). Other chromosome numbers were seen, mostly at very low frequencies, except for the count of 2111s found in 37.10% of observed cells in M. cumingii. Relatively normal behavior of chromosomes was found in all stages of meiosis for M. cumingii, although abnormalities, such as laggards (up to 20.00%) and bridges (4.29%) were also observed. Both M. pendula and Medinilla sp. showed abnormal meiosis, having high frequencies of laggards (up to 29.03% and 43.08%, respectively), bridges (16.13% and 14.29%, respectively), and non-congression (up to 50.75% and 62.69%, respectively). Pollen fertility was high in M. cumingii (91.63%), which could be attributed to a relatively normal behavior of dividing cells at all stages of meiosis. Both M. pendula and Medinilla sp. exhibited low pollen fertility at 25.24% and 24.06%, respectively, possibly due to a relatively high percentage of cells with abnormalities.

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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