Screening and characterization of bacterial antagonists for the biological control of damping- off and stalk rot of corn caused by Fusarium verticillioides (Sacc.) Nirenberg 1976

Date

4-2013

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Marina P. Natural

Abstract

Bacteria were isolated and screened for antagonism against Fusarium verticillioides. Their potential for biological control of damping-off and stalk rot of corn was evaluated. Out of 100 bacterial isolates, 23 isolates showed antagonism against F. verticillioides in multiple cultivation assays. After conducting final screening in dual culture, three (3) best performing isolates were selected. Selected antagonists (CSA5, QR1 and CSB1) were further evaluated in vitro and in vivo. Dual culture assay showed that all isolates inhibited mycelial growth ๐‘‘ž% (p0.05). In vivo results showed that significant reduction of damping-off incidence was achieved with seeds coated with bacterial cells of isolates CSA5 and CSB1 compared with the untreated seeds. In stalk rot trials, highest reduction of disease severity were observed with CSA5 and CSB1 treated plants (p0.05). Isolate QR1 showed the weakest ability to overcome the disease in both trials. Based on cultural and biochemical characterization, genus of CSA5, CSB1 and QR1 were presumptively identified as Bacillus sp., Lactobacillus sp. and Bacillus sp., respectively. Results of this study revealed the potential of CSA5 and CSB1 as promising biocon agent of damping-off and stalk rot of corn caused by F. verticillioides.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2013 A3 M365

Document Type

Thesis

This document is currently not available here.

Share

COinS