Morphological characterization and isozyme analysis of six banana (Musa sp.) cultivars.

Date

3-2002

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Felipe S. Dela Cruz Jr.

Co-adviser

Merlyn S. Mendioro

Abstract

Six banana cultivars, namely: Balatay, Bataan, Dumanese, Matavia, Saba, and Tiparot were characterized using morphological evaluation and isozyme analysis. There were 18 invariant characters observed in plant habit and bunch and fruit characters. The dendrogram generated using the qualitative morphological characters showed that Balatay and Tiparot (ABBB) group together, Saba (BBB/BB) with Dumanese, while Matavia and Bataan did not group neither with the two clusters. Two clusters were observed from the dendrogram generated using the quantitative data. The first cluster included Balatay, Matavia (ABB) and Tiparot (ABBB) while the second cluster consisted of Bataan, Dumanese and Saba (BBB/BB). Both dendrograms resulted to the clustering of Balatay with Tiparot (ABBB) and Dumanese with Saba (BBB/BB). Isozyme analysis revealed that Matavia (ABB), Saba (BBB/BB), and Tiparot (ABBB) had the same banding patterns for MDH, PGD and PGI, thus no specific genome banding pattern was observed to which the cultivars with unknown genomes can be compared to. For PGM, only Matavia showed a different banding pattern. Thus, the isozyme systems used were not effective in classifying the cultivars under their possible genomic group. However, based on their isozyme profiles Balatay, Bataan, and Dumanese were three different cultivars. More enzyme systems must be used or more powerful molecular techniques must be employed for genome determination.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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