Agro-morphological and cytogenetic analyses of rice (Oryza sativa L.) IR64, SGR 652 (Golden Rice) and their F1 hybrids and detection of crtl gene in the F2 using polymerase chain reaction.

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Twenty-two agro-morphological traits of rice, 0. saliva L. (elite IRRI bred indica cultivar IR64, transgenic javanica variety Golden Rice SGR 652 and their F1 hybrid), namely, blade pubescence, blade color, ligule shape, apiculus color, basal leaf sheath color, blade angle, ligule color, collar color, culm angle, internode color, flag leaf angle, panicle exsertion, panicle axis, awning, stigma color, sterile lemma color, leaf length, leaf width, culm length, culm number, panicle exsertion and % grain filling were described and compared. Of the 16 qualitative traits, 12 traits were the same in all plant materials. Two characteristics similar in the Fi hybrid and IR64 were blade pubescence and blade color while the two characteristics similar in the F1 hybrid and SGR 652 were ligule shape and apiculus color. Using LSD, it was determined that the F1 hybrid had a different mean from the parents for leaf length, leaf width, and culm number. For ruins and panicle number, the F, hybrid had a different mean from SGR 652. All means were different in the three plant materials for percent grain filling. A large variation ranging from 2n=20 to 2n=26, with peak at 2n=24, was observed in the chromosome numbers of IR64, SGR 652, and this F1 hybrid. The Fi hybrid showed the lowest percentage (77.89%) of cells with 2n=24 chromosome number. More variations in chromosome number were observed in the hybrids compared to the parents. Meiotic observation at Metaphase I of IR64, SGR 652, and their Ft hybrid showed majority of cells were normal. The most common abnormality observed in all plant materials was early disjunction. Other abnormalities observed in the F1 hybrid were slanted spindle fibers, and non-congression. Sticky chromosomes were observed in IR64. At Anaphase I, bridges and laggards were observed in SGR 652 while only bridges were observed in IR64. Late disjunction was also observed in IR64. Pollen fertility was very low in the F1 hybrid (6.8%). This may be because the F1 as a cross between intervarietal group indica (IR64) and javanica (SGR 652). The ert/ gene was detected in nine out of 20 F2 plants using PCR. This suggests that the F1 hybrid inherited the inserted gene from SGR 652 and was able to pass it on to the F2 population.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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