"Preliminary cytogenetics of two predatory mites Amblyseius calorai Corpuz and Rimando and Amblyseius lenis Corpuz and Rimando (Acarina : Phytoseiidae)."

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

Adult species of predatory mites, Amblyseius calorai Corpuz and Rimando and Amblyseius lenis Corpuz and Rimando were collected from mass-reared cultures, fixed in Farmer's solution and stained with lacto-aceto-orcein. Meiotic stages observed for A. calorai and A. lenis were Prophase I and Metaphase 1 stages only Their meiotic indices were 28.18% for A. calorai and 29.66% for A. lenis, having Prophase as the dominant stage for the both species. Results showed that A. calorai have a chromosome number of 2n=18 based on the Pachytene stage and 2n=16 from Diplotene stage. While A. lenis has a chromosome number of 2n=18 based on its Pachytene stage. Lengths of the Diplotene stage and Pachytene stage chromosomes of A. calorai ranged from 2.696 p to 4.628 p and 1.026 p to 8.002p respectively. Relative lengths of Pachytene stage chromosomes ranged from 0.128 to 1.000. The d-value was from 0.237 to 5.396, while their r-value ranged from 1.490 to 5.11, respectively. Chromosomes were monocentric and were classified as subterminal (chromosome I ), submedian (chromosome 4, 5. 6, and 7), and the rest were all median. The relative lengths of the Diplotene chromosomes varied from 0.583 to 1.000. The chromosomes based on their centromeric positions were generally median in classification. Three chromosomes (1, 3, and 5) were submedian. The chromosomes of A. lenis were generally subterminal in classification. Three chromosomes (1, 2, and 9) were median. While, chromosome 7 is submedian. They were also monocentric. The lengths of A. lenis Pachytene stage chromosomes ranged from 3.267p to 10.181p, with relative lengths of 0.321 to 1.000. Based on the computed relative lengths of the Pachytene stage of both species, A. lenis has longer chromosomes and has more variation in their length than in A. calorai.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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