"Relationship of papain to nucleic acids in the sap of Carica papaya Li" by Alma A. Cruz

Relationship of papain to nucleic acids in the sap of Carica papaya Linn.

Author

Alma A. Cruz

Date

3-1986

Degree

Bachelor of Science in Biology

Major Course

Major in Ecology Genetics

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Saturnina C. Halos

Abstract

An investigation designed to determine the relationship of papain content to nucleic acids in the sap of papaya fruits was conducted. The effect of fruit ripening on papain content and nucleic add content of the plant sap system was likewise deter-mined to evaluate possible control mechanisms of protein biosynthesis. The latex yields of the ripe fruits were found to be 24.72% and 20.34% only of the latex yields of the green fruits for the two trees respectively. Crude papain yields of ripe samples were respectively 59.58% and 60.97% of the corresponding green samples. Total protein content of the crude enzyme extracts were also found to be lower in ripe samples. Even the relative activity of the samples from the ripe fruits were found to be slightly lower. All samples possess only one kind of nucleic acids as shown by the single bands. The nucleic acids of the ripe samples were found to have higher relative mobilities implying lower molecular weight. The data infers that the nucleic acids could be tRNA's. The study failed to show that there is a probable relationship between the papain production and the acids found in the sap of the papaya fruits.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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