Isozyme and hemocyte analyses of populations of the Europian honeybee, Apis mellifera Linnaeus, reared in agricultural, forested, and industrial areas.

Date

4-2001

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Lynn S. Villafuerte

Abstract

The changes in the hemocytes and isozymes in populations of European honeybees, Apis melYera Linnaeus reared in three different areas, namely: Calauan (agricultural), Forestry (forested), and Canlubang (industrial) were determined via microscopic observations for hemocyte analysis and electrophoresis for isozyme analysis. Results in hemocyte analysis showed that prohemocytes and granulocytes were present in A. mellifera Linn. There was no significant difference observed in the total hemocyte counts (THC's) in honeybees among the three different areas except in the unmanaged colonies after transport. Significant differences were observed between the frequency of prohemocytes and granulocytes (DHC's) in the control populations (before transport). There was an increase in prohemocytes in the experimental group (after transport) especially in the unmanaged colonies in Canlubang. In the isozyme analysis, alkaline phosphatase (ALPH), acid phosphatase (ACPH), and malic enzyme (ME) were observed to be polymorphic in all the populations studied. Analysis of this showed that the type of area can affect the genotype and gene frequencies. Comparison of the areas showed the highest genetic identity before and after was 1.1014 and 0.6426, which was between the colonies in Canlubang and in Forestry and between the colonies in Canlubang and Forestry, respectively. The lowest values obtained before and after transport was 0.4013 and 0.2251, which was both between the colonies in Canlubang and in Calauan. No significant differerences were observed between the managed and unmanaged populations.The high genetic values suggest that the populations were still genetically similar despite transport and rearing in three different areas.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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