Genetic relationships in the Solanum and Capsicum germplasm collection of the Asian Vegetable Research and Development Center (AVRDC), Tainan, Taiwan based on conserved orthologous set II markers

Date

4-2007

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Dennis B. Bela-Ong

Abstract

Conserved Orthologous Set II (COS II) markers were used to determine the genetic relationships among species of Solanum (subgenus Leptostemonum and Potatoe) and Capsicum. Ninety-six accessions were used in the study (35 Lepto\temontun 34 Potatoe, and 27 Capsicum). Among the 66 COS H markers that were screened. 22 with highly resolved bands were used for the inter-accession study. These COS II primers amplified 86 distinct bands with a range of 2-9 per primer. Only one primer, C2_At2g47580, showed a monomorphic band for all the accessions. NTSYS was used to construct a dendrogram by first calculating for the Jaccard's similarity coefficient. Four clusters resulted from the analysis showing segregation of the plant taxa. Cluster I included all the Solanum subgenus Leptostemonum species except ferox and stramonifolium with similarity value of 84%. Cluster 2 has the highest similarity (92%) and is composed of the Solarium subgenus Potatoe. Cluster 3 is composed of S ferox and stramonifolium with similarity value of 80%. The last cluster is composed of all Capsicum species (similarity = 84%). Results of this study show that COS II markers can be a powerful tool in assessing genetic relationships between plant species and genera.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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