Genetic variation in two local populations of the sweet potato weevil, Cylas formicarius, Fabr. (Colioptera : Curculionidae)

Date

3-1992

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Presidena A. Baculod

Abstract

Three enzymes were investigated by starch gel electrophoresis for genetic variable in different developmental stages of sweet potato weevil. Cylas formicarius Fabr. from Batac and Los Banos. Esterase was found to be dimorphic, with bands est-A and est-B exhibiting mean Ef values of 0.39 and 0.36, respectively. Among the three phenotypes observed in all stages, phenotype 2 was relatively high in both populations than phenotypes I and 3. The adults also exhibited a relative decrease in the frequency of the two esterases. Acid phosphatase was monomorphic (AA) which only the band and a mean Rf value of 0.32. The band has a 100% frequency in all stages except in the pupa in which 47% and 57% frequency was observed in Batac and Los Banos populations, respectively. Alkaline phosphatase was dimorphic, with bands A1Ph-A and A1Ph-B showing mean RF values of 0.33 and 0.23, respectively. A1Ph-A was observed in all stages whereas A1Ph-B was active only in the first larval instar and in the adult stages of both populations. Of the three phenotypes observed, phenotype 2 was relatively higher in frequency than phenotypes 1 and 3 in Batac populations of the SPW while it was reversed in Los Banos populations. Heterozygosity of the two populations in the esterase locus was not significant at 5% level while the A1Ph locus showed significant heterozygosity. Both populations also showed significant deviations from the Hardy-Weinberg equilibrium.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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