Studies on the development of the coconut water as a cell culture nutrient medium for embryonic chick cells

Date

10-1978

Degree

Bachelor of Science in Biology

Adviser/Committee Chair

William G. Padolina

Abstract

Cells, obtained from the trypsinization of whole ten day old chick embryos, were cultured in coconut water nutrient medium within a sixty-eight hour period, using coconut water from young, three month old coconuts. The embryonic chick cells were cultured in five sterile flasks containing coconut caster medium (experimental medium) and in five sterile flasks containing distilled water (control medium), all of which were maintained at 38,5°C in an incubator. Cell counts, morphological observations, and pH approximations of media, done every twelve hours, showed normal growth curves of the cell populations, and growth rates manifesting mitotic activity. Nutrient supply, pH, accumulation of metabolic wastes, and microbial interference at the 68th hour were found to be various environmental limiting factors for continued population growth of cells. The study supports the hypothesis that coconut water can nourish and stimulate the growth of mitotic chick cells. Further chemical studies and biological assays on the growth promoting effect of coconut water on other animal tissue cells, as well as chemical modifications of the medium, is recommended.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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