Preliminary study on the effect of Gamma-BHC on liver mitochondrial succinate dehydrogenase activity in Guinea Pig (Cavia sp.)

Date

3-1976

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

Mitochondria from guinea pig liver were treated with I -BHC both in vivo by force-feeding the animals, and in vitro. The rate mitochondria succinate dehydrogenase activity was measured using the DPIP dye-reduction technique. In vitro, addition of j-BHC produced a marked inhibition of succinate dehydrogenase with the degree of inhibition increasing with the dosage of j -BHC applied. The probable cause of such inhibition is the penetration of the lipid-soluble j -BHC into the mitochondrial membrane, and the subsequent disruption of the integrity of the protein structure necessary for maximum catalytic activity. In vivo studies showed an enhancement of oxidative activity possibly due to the presence of factors in the intact animal which divert or block the insecticide's inhibitory effect.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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