Morphological characterization and isozyme analysis of different accessions of sweet potato (Ipomoea batatas L.)

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

Morphological characterization and isozyme analysis were conducted on 100 accessions of sweet potato (Ipomoca bulutas L.) obtained from the collection being maintained at National Plant Genetic Resources Laboratory, Crop Science ('luster-Institute of Plant Breeding. Vine, Leaf and storage root qualitative morphological characteristics such as vine color, pubescence, leaf shape, leaf type, leaf color, flesh and skin color of storage root revealed diversity among accessions of sweet potato. A wide diversity was also observed from the constructed dendrogram using NTSYSpc by SAHN clustering. At a similarity index of 0.53, accessions failed to group into common clusters indicating wide morphological diversity. Isozyme analysis yielded five putative loci encoding three enzymes. Three zones of activity were observed for esterase (EST), and one locus for both alkaline phosphatase (ALP) and acid phosphatase (ACPH). EST-3, ALP-I and ACP-I were loci were found to be polymorphic. Fifteen accessions were distinguished based on unique banding patterns for EST (PHL8516), ALPH (Acc493 and Acc664) and ACPH (12 accessions). The dendrogram constructed revealed greater similarity among accessions. Four clusters were obtained at 0.69 index of similarity. Also, accessions collected from the same locations grouped under common clusters.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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