Single cell protein production by Cellulomonas flavigena ATCC 15599 using molasses as carbon source

Date

10-1984

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Asuncion K. Raymundo

Abstract

A cellulolytic bacterium, Cellulomonas flavigena ATCC 15599 was used for SCP production using molasses at different levels of sugar concentration as the sole carbon source. Using shake-flask method at ambient room temperature, the microbial growth reached a maximum at 48 hours. The highest cell density was observed to be 2.99 x 108 for 1% sugar level from molasses and 3.0 x 109 cells per ml for K. Values did not differ significantly from each other. However, differences in maximum cell mass yields were significant with 5.0 g/1 for 1% sugar level from molasses and 5.32 g/1 for 2% level. A gradual decrease in pH was observed in both levels. Residual sugars of 1.45 mg/ml and 2.30 mg/ml were obtained in media with 1% and 2% total sugar concentration of molasses, respectively, after. 96 hours fermentation. Under the conditions of the study and without pH control, a 1% total sugar concentration of molasses was considered optimum for the growth of Cellulomonas flavigena.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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