Molecular mapping of grain quality quantitative trait loci in rice (Oryza sativa L.) by selective genotyping using simple sequence repeats
Date
3-2003
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Merlyn S. Mendioro
Abstract
Ten grain quality characters in rice (00,:a saliva I were used to characterize two-advanced backcross inbred lines. RF52 (1R64 x Karnal Local) and RF57 (I cutting x l3asmati). Results of the phenotypic data analysis show that RF52 has intermediate amylose content (amy), soft gel consistency (gel con). intermediate gelatinization temperature (gela), moderate aroma, very long cooked kernel length (c length), short cooked kernel width (c width), slender cooked length-width ratio (c 11W). long uncooked kernel length (un length), short uncooked kernel width (un width) and slender uncooked length-width ratio (un L/W). On the other hand. RF57 showed the following grain quality characteristics: intermediate amy, medium gel con, intermediate gela. slight aroma. very long c length. short c width, medium c L/W, intermediate un length, short an width and medium un L/W. Based on correlation analysis, un L/W & un length (R1252) and c L/W & c length (RF57) show very strong positive linear association. A very strong negative linear association is exhibited by c L/W & c width (RF52). Both from RF57. c L/W & c width and un L/W & un width showed strong negative linear association. Furthermore. two correlation pairs from RF57 showed moderate positive linear association-- c L/W & gela and un length & c length. On the other hand, tin L/W & tin width (RF57) showed moderate negative linear association. Sixty-nine putative QTLs controlling grain quality traits were detected. 42 for RF52 cross and 27 for RF57 cross. These QTLs are widely distributed over the 12 chromosomes of rice and together explains the phenotypic variation for the analyzed grain quality traits in the two populations. QTLs for amy were located in chromosomes 3 & 5 (R.F52) and 8 (RF57). Three QTLs each for gel con were identified in chromosomes 2 and 11 for the two crosses and the rest were identified its chromosomes 3. 4. 6 (RF52) & 5 (RF57) controlling gel con. QTLs for gela were located in chromosomes 1. 2. 4. (RF52) 2, & 8 (R1'57): for aroma in 1, 7, 8 (RF52) 9, 10. & 11 (RF57); for e length in I. 4. 5. 10 (RF52) 2. 5. 7, &12 (RF57); for c width in 5, 6. 7, 8. 10, 11 (RF52) & it) 11(1:57): for c L/W in 5. 6, 7, 8. 1(), 11 (RF52) & two in 7 and one in 12 (RF57): for in length in I. 6. 9. 11, 12 (RF52) 2. 5. & 10 (RF57); for un width in 3, 4, 8. 10. II (R L52) 2. 6. 8. & 11 (RF57); and for un L/W in 6, 9. (RF52) 2, & 6 (RF57).
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Mojica, Julius P., "Molecular mapping of grain quality quantitative trait loci in rice (Oryza sativa L.) by selective genotyping using simple sequence repeats" (2003). Undergraduate Theses. 10991.
https://www.ukdr.uplb.edu.ph/etd-undergrad/10991
Document Type
Thesis
