Genetic characterization of the physic nut, Jatropha curcas L., through isozyme analysis

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Adelina A. Barrion

Abstract

The extent of genetic variability of the physic nut, Jatropha curcas L., was assessed and characterized in a representative set of 96 accessions collected from 16 provinces in the Philippines. Using starch-gel electrophoresis and enzyme systems such as acid phosphatase (ACP), alkaline phosphatase (ALP), and esterase (EST), ten presumptive loci were identified: three loci each for ACP and ALP and four loci for EST. Codominant alleles S (slow) and F (fast) coded for ACP-2, ALP-3, EST-1, and EST-2 while only the S allele controlled ACP-1, ACP-3, ALP-1, ALP-2, EST-3 and EST-9. Proportion of polymorphic loci (P), average number of alleles per locus (A), and heterozygosity over all loci (H) were relatively low (0.40, 1.4, and 0.17, respectively) which indicate low genetic variability among the 96 accessions of J. curcas. Cluster analysis was performed by constructing a dendrogram which generally grouped the accessions based on their geographical location. This geographical differentiation of J. curcas could be attributed to differences in environmental conditions among various provinces.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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