Electrophoretic analysis of soluble proteins, glucose-6- phosphate dehydrogenase and esterase isozymes of different Azolla pinnata B. accessions.

Date

3-1990

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Co-adviser

Presidenna A. Baculod

Abstract

General proteins, glucose-6-phosphate dehydrogenase (G6PD) and esterase isozymes of eight accessions of Azolla pinnata were studied using polyacrylamide disc gel electrophoresis. The protein test revealed a total of eight bonds which have variable Rf values, band patters, and bond intensities. On the other hand, esterase isozymes revealed a total of six bands and these bands have a regular banding pattern, Rf values and the band intensities are consistent with the pattern. Band patterns and similarity index revealed genetic difference for all accessions in terms of general proteins. For G6PD, no bands were revealed, instead white bands were noted. Minor genetic differences were noted for esterase. The minor variations observed in esterase isozyme and the consistency of the band pattern and intensity for all the accessions qualified it as a possible biochemical marker for the Azolla pinnata accessions studied.

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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