Genetic diversity in Solanum species from Southeast Asia based on SDS-page of seed proteins

Date

10-1999

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Nerissa K. Torreta

Co-adviser

Liwayway M. Engle

Abstract

This study was conducted to determine the degree of diversity among different species of Solarium collected from Southeast Asia, namely: S. aculeatissimum, S. aethiopicum, S. incanurn, S. indicum. S. rnacrocarpon, S. melongena, S. nigrum, S. parlcinsonii, S. pectinatum, S. pseudocapsicum, S. stramonifolium, S. surattense, S. torvum and S. xanthocarpum using SDS-PAGE of seed proteins. Specifically, the study determined the SDS-PAGE seed protein bands and banding pattern in 14 Solarium species collected from Southeast Asia, determined the geographic distribution of the bands and band patterns, calculated the relative frequency of occurrence of specific bands within species, calculated the similarity index between 14 Solarium species based on SDS-PAGE seed protein bands and inferred some taxonomic relationships in the 14 Solarium species of Southeast Asia. Sixteen major bands were resolved by SDS-PAGE. Band 3 was the most frequent band occurring in 79 of 100 Solarium accessions studied. Band 2 was the least common band. It was noted only in S. indicum and S. stramonifolium. Thirty-one protein band patterns (BP) were exhibited by the Solarium accessions. Band pattern 8 was the most common band pattern having occurred in 16 % of all Solarium accesssions studied. Band patterns 3, 9, 16, 22 and 27 were found to be specific to S. aculeatissimum, BP 24 to S. indicum, BP 30 to S. nigrum, BP 4, 11 and 21 to S. torvum and BP 7 and 31 to S. xanthocarpum. A specific band pattern predominated in each country of origin. BP 8 predominated in Malaysia and Thailand and BP 17 in the Philippines. The most frequent band occurring in all countries was BP 14. However BP 11, 17 and BP 31 were the only band patterns not found in Thailand. Hence, the center of diversity among the three Asian countries was Thailand. Similarity index values showed that interspecific variation is greater than intraspecific variation among Solarium species. The taxonomic relationship of the 14 Solarium species was inferred based on similarity index value and average-linkage dendogram obtained through cluster analysis. S. melongena was found to be most closely related to S. aethiopicum, followed by S. macrocarpon, S. pectinatum, S. stramorufoliurn, S. torvum, S. xanthocarpum, S. nigrum, and relatively distant to S. aculeatissimum and S. indicum. This result is strongly supported and agrees with that of serological studies, crossability and hybridization experiments, chloroplast DNA analysis, allozyme analysis and electrophoretic data. The SDS-PAGE protein profile, that confirms known morphological data, can determine the genetic base of the 14 Solarium species. With this, SDS-PAGE can be a useful tool in characterizing genetic diversity since it is less expensive and easy to implement.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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