Callus induction in leaf explants and subsequent organ differentiation in two cultivars of sweet potato (Ipomoea batatas L. Poir.) on different culture media

Date

3-1992

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Alfinetta B. Zamoran

Abstract

Leaf sections measuring about 1 mm x 1 mm from the first expanded leaves of shoot cultures of two cultivars of sweet potato (Ipomoea batatas L. Poir.). namely PNG-L318 and PNG-L6. were introduced in vitro culture to form callus that subsequently differentiated on different culture media. The best callus initiation medium for both cultivars Was a modified Murashige and Skoog's medium (MS-Mod) supplemented with 0.03 ppm NAA and 0.3 ppm BA (LRM) which induced compact and globular calli that were capable of organ differentiation when transferred to appropriate regeneration media. The LRM-induced calli of both cultivars respond differently to the culture media. In PNG-L318. root differentiation was obtained on MS + 2 ppm NAA + 0.2 ppm BA whereas ln PNG-L6 roots were obtained on MS + 1 ppm IAA + 1 ppm Xi. MS + 0.2 ppm IBA + 0.2 ppm BA and MS + 2 ppm NAA + 0.2 ppm BA.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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