Verification of interspecific hybridity of Allium cepa L. x Allium fistulosum L. using Random Amplified Polymorphic DNA (RAPD)

Date

3-2000

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Marcelo A. Duka

Abstract

This study was conducted to verify the interspecific hybrids of Allium cepa L. and Allium fistulosum L. using Random Amplified Polymorphic DNA (RAPDs). Polymorphic RAPD markers of the parents were identified. RAPD was evaluated as a suitable technique to carry out this study. Forty primers were used, OPAX 1-20 and OPAT 1-20, from which thirty-one primers generated good bands. Out of the thirty-one primers, only thirteen showed polymorphism between the parents of the five crosses. These were then used to verify the progenies of each cross. All the progenies proved to be hybrids. CF54 has twenty-six bands, fifteen came from the female, nine from the male and two were unique. FC45 has sixty total bands, twenty four are from the female and the remaining thirty six came from the male. FC46 has fourteen bands, eight from the female and six from the male. CFI9 has fitly-one, eighteen from the female, twenty-eight from the male and five were unique. CFI has sixty-two bands, twenty-four from the female, thirty-three from the male and five were unique. Lastly, CF 16 has twenty bands which are equally from both parents. Horticultural characteristics showed that the progenies are intermediate of their parents. These results proved that RAPD could be a suitable method in verifying interspecific hybridity between the two allium species.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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