Comparative karyotypes of eggplant fruit and shoot borer, Leucinodes orbonalis Guenee (Lepidoptera: Pyralidae), from Laguna, Leyte, and Pangasinan.

Date

4-2012

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Cheryl A. Agdaca

Abstract

The mitotic indices, chromosome numbers, and relative chromosome lengths of the eggplant fruit and shoot borer (EFSB), Leucinodes orbonalis Guenee, from UPLB Laguna, Baybay, Leyte, and Sta. Maria, Pangasinan were compared. Squash preparations from the salivary gland in the anterior portion of five third instar larvae from each location were prepared using the lacto aceto-orcein squash technique. Based on the frequencies of the mitotic stages during the developmental stage used, the cells stay longer at prophase wherein it had the highest percentage for all of the EFSB populations with 60.96 %, 75.40 %, and 76.38 % for Laguna, Leyte, and Pangasinan, respectively. The highest computed mitotic index was 37.12 % for the Pangasinan population, followed by Leyte with 34.77 % and Laguna with 25.48 %. This may suggest that at the third instar larval stage, relatively low proportion of cells enter mitosis and eventually complete the cell cycle. Variations in the number of chromosomes were observed. The modal diploid chromosome number of EFSB from Laguna, Leyte, and Pangasinan populations were 2n:=36, 2n=32, and 2n=30, respectively. Karyotypes showed small spherical chromosomes with relative length ranges of 0.0406-0.0699, 0.0357-0.0859, and 0.0483-0.0839 for Laguna, Leyte, and Pangasinan, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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