Effects of different culture media, length of storage, and size of seed explants on the in vitro embryo culture of immature yard-long bean,Vigna unguiculata Walp. ssp. sesquipedalis (L.) Verdc.

Date

4-2005

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Factors responsible for the successful in vitro regeneration of embryo from immature yard-long bean (Vigna unguiculata Walp. ssp. sesquipedalis (L.) Verde.) collected from Shanhua local market in Tainan, Taiwan were determirH by measuring the number of leaves, plantlet height, number cf roots, root length, percent survival, and percent contamination. Eight treatment combinations were formulated based on the following factors: different culture media - Murashige and Skoog (MS) medium or MS with indole-3-acetic acid (IAA), size of seed explant — whole or half seed, and height of storage - 0-1 day or 7-8 days. Yard-long bean cultured in either MS or MS + IAA media, stored for 7-8 days, and using either whole seed or half seed explants had significantly more number of leaves at the 3" and 6th day after inoculation (DAI) (1-3 leaves). On the 9th a 12th DAI, Tukey's studentized range test or the honestly significant difference (IISD) test showed that only the whole seed explants stored for 7-8 days and cultured in either type of culture media had significantly higher number of leaves (2-4 leaves). Storage had the same effects on the plantlet height and percent contamination, i.e. yard-long bean cultured in either type of media and explant that were stored for 7-8 days significantly produced taller (.1.0 cm — 9.4 cm) and higher number of contaminated plantlets (10 — 100 percent). However, all factors had insignificant effects on the number of roots, root length and percent survival. Regenerated yard-long bean plants were subjected to hardening procedures at various rooms of acclimatization (24 + 1 °C, 28 + 1°C, and 32 + 1°C). The tallest plants (11.9 cm) were observed from transplanted regenerates previously cultured at MS media, stored for 0-I day using whole seed explants. The mean number of leaves for each treatment combination significantly varied (0-8 leaves). Results indicated insignificant effects of factors on yard-long bean in transplant, therefore, the effects of different culture media, length of storage and size of seed explants in vitro play a crucial role in determining the vigor of plants in transplant and must be considered in ',he in vitro embryo culture of immature yard-long bean.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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