An exploratory study on the direct DNA synthesis through the detection of Acyl Carrier Protein (ACP) gene using the water from coconut (Cocos nucifera L.).

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Co-adviser

Marni E. Cueno

Abstract

To directly synthesize cDNA using the mRNA in the DNA-mRNA hybrid in coconut water through reverse transcription, free nucleic acids (FNA) were first isolated from the water of coconut (Cocos nucifera L.) with leaf rank (LR) 15 and 18, and were used directly for cDNA synthesis. The DNA-mRNA hybrid was used as a template for reverse transcription following the protocol for transcripts with high GC content. Amplification of cDNA was done through cold-start PCR and analyzed using Agarose gel electrophoresis. The cDNA was tested through the detection of the acyl carrier protein (ACP) gene. No bands were generated in both samples of the RACE product, and only primer-dimers were obtained. Traces of RNA were obtained from the cDNA products of both samples and no cDNA bands were produced. The absence of a RACE product and presence of RNA suggest that cDNA was not synthesized. Reverse transcription did not occur, thus no cDNA was obtained. The reverse transcriptase enzyme activity was hindered, which could possibly suggest that the template remained double stranded, thus the mRNA may not have separated from the DNA-mRNA hybrid.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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