Electrophoretic analysis of soluble proteins, glucose-6- phosphate dehydrogenase and esterase isozymes in six accessions of Azolla pinnata var. pinnata Lam. (Salviniales : Azollaceceae)
Date
3-1992
Degree
Bachelor of Science in Biology
Major Course
Major in Systematics
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Presidena A. Baculod
Abstract
Polyacrylamide disc gel electrophoresis was used to investigate similarities and differences among pinnata var. kinnata accessions 7001, 7002, 7003, 7004, 7016 and 7017. The general proteins revealed a total of 10 bands which were divided into four zones. These four protein zones were present in the different accessions except for 7002 which has no band in zone 1. The general proteins revealed significant differences among accessions based on relative electrophoretic mobilities as well as the number of bands for each accession - 7001 had 7 bands, 7002 had 6 bands and the other four accessions had 6 bands. Five bands were present in all accessions but have varying intensities. Relative electrophoretie mobilities of esterase showed no significant difference among accessions. Only one phenotype, with a total of four bands, was observed in all accessions with small and insignificant differences in Rf values. The esterase isozymes had a regular banding pattern and band intensity. For Glucose-6-phosphate dehydrogenase, no bands were rev,7).led. Instead, white bands were observed. This negative staining was also noted in previous study.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Flores, Jose M. Jr, "Electrophoretic analysis of soluble proteins, glucose-6- phosphate dehydrogenase and esterase isozymes in six accessions of Azolla pinnata var. pinnata Lam. (Salviniales : Azollaceceae)" (1992). Undergraduate Theses. 11073.
https://www.ukdr.uplb.edu.ph/etd-undergrad/11073
Document Type
Thesis
