Molecular analysis of elite introgression lines from the cross of rice (Oryza sativa L.) and a wild species (O. longistaminata A. Chev. et Roehr.) using Simple Sequence Repeat (SSR) markers

Date

4-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn O. Villa

Co-adviser

Darshan S. Brar

Abstract

FRANCISCO, ANCHILIE GAMBOA. 2009. Molecular Analysis of Elite Introgression Lines Derived from the Cross of Rice (Oryza saliva L.) and a Wild Species (0. longistaminata A. Chev. et Roehr.) Using Simple Sequence Repeat (SSR) Markers. University of the Philippines Los Banos. B.S. Biology Major in Cell and Molecular Biology. Adviser: Prof. Neilyn 0. Villa (UPLB) Co-adviser: Dr. Darshan S. Brar (IRRI) ABSTRACT Ten alien introgression lines (AILs) selected from the cross of New Plant Type (NPT) of rice (Oryza saliva) and a wild species (0. longistaminata) were used in the analysis of yield and yield components. Significant differences were found for number of tillers per plant, plant height, panicle length, grains per panicle, 100-seed weight and grain yield per plant. The introgression lines were superior to the recurrent parent. A set of 66 SSR markers distributed on 12 chromosomes was tested and showed 25.76% introgression. Furthermore, AIL 1, 2, 3, 6, 7, and 10 have introgression in chromosome 1 specifically within the range (66.2-115.2 cM) of yield enhancing loci, yld1.1. These lines showed a significant increase in the number of tillers per plant, panicle length, 100-seed weight and grain yield per plant. Thus, wild species of rice particularly 0. longistaminata, though phenotypically inferior, carry some useful alleles that can improve yield and yield components of rice varieties.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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