Genetic diversity of the elite lowland irrigated varieties of Rice (Oryza sativa L.) grown from 1976 to 2005 based on Simple Sequence Repeat (SSR) markers

Date

4-2008

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Cecilia S. Reamillo

Co-adviser

Edilberto D. Redoña

Abstract

GALELA, HEDY S. 2008. Genetic Diversity of the Elite Lowland Irrigated Varieties of Rice (Oryza saliva L.) Grown from 1976 to 2005 Based on Simple Sequence Repeat (SSR) Markers. University of the Philippines, Los Banos. B.S. Biology major in Cell and Molecular Biology. Adviser: Prof. Ma. Cecilia S. Reamillo Co-adviser: Dr. Edilberto D. Redona

ABSTRACT

Data on the most phenotypically acceptable rice varieties grown in the International Irrigated Rice Observational Nurseries (IIRON) were obtained and subjected to frequency counting across test locations and across years. Genetic diversity analysis using simple sequence repeat (SSR) markers and multivariate analysis using MANOVA were conducted among the thirty rice accessions available at the International Network for the Genetic Evaluation of Rice (INGER). The 47 polymorphic SSR markers used in the study revealed a total of 216 alleles with an average of 4.60 alleles per locus. Among these markers, a total of 15 rare alleles were also detected. The polymorphism information content (PIC) values obtained ranged from 0.1308 to 0.8556, with an average of 0.63 per marker. Overall genetic diversity was also calculated using Shannon's diversity index, which was found to be equal to 0.65. Cluster analysis of the varieties allowed identification of two clusters at approximately 45% level of similarity, and four subclusters were identified at 54% level of similarity. The varieties were found to be mostly clustered according to subspecies and country of origin. MANOVA was conducted to detect correlations among SSR variation, plant height, grain yield, amylose content, average vegetative vigor and days to flower. A slight positive correlation was established between SSR variation to plant height, amylose content and average vegetative vigor.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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