In Vitro, culture of mouse skeletal muscle cells in coconut water media

Ruth O. Gapaz

Date

3-1982

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

ABSTRACT

GAPAZ, Ruth Ona. 1982. In Vitro Culture of Mouse Skeletal Muscle Cells in Coconut Water Medium. An undergraduate thesis submitted in partial fulfill ment of the requirements for the degree of Bachelor of Science in Biology, under the a6visorship of Dr. William G. Padolina. University of the Philippines at Los Banos. Second Semester. 1981-1982.

Cells obtained from the mechanical and matic dispersion of skeletal muscles from one-day-old mice were cultured at 38.5 C for ten days in increasing volumes of coconut water extracted aseptically from nine month old coconuts. Statistical analysis using RCB design and HSD test at l and 57', level of significance reveals that coconut water medium like Eagles Minimum Essential Medium,(a highly recommended medium) c nourish and stimulate the growth of mitotic mic skeletal muscle cells. As exhibited by normal growth curves of the cell culture populations that were observed for all treatments(0% CW, 25%CW, 5CY: CW,10D CW) within a period of ten days, all the treatments manifest significant mitotic activities after three days and continued exponential phase up to eight days of incubation period. Morphological and dye exclusion indices supported by statistical analysis indicates that cocowater performs even better than 75% coconut water and 100% coconut water. This is mainly due to pH stabilization in 0% CW,25CW, 50% CW and lowered pH of 75% CW( at eighth day of incubation) and 100% CW( at seventh day of incubation).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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