A comparative electrophoretic study of the Philippine milkfish, Chanos chanos (forskall) (Osteichthyes: Chanidae) raised in two freshwater sites.

Date

5-1986

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

ABSTRACT GARCIA, MABEL B., University of the Philippines at Los Banos, May, 1986. A Ccmparative Electrophoretic study of the Philippine Milkfish, Chanos chanos (Forskall) (Osteichthyes:Chanidae) Raised in Two Freshwater Sites. Major Adviser: Mr. Ivan-Marcelo A. Duke

Blood samples from 56 milkfish (Chanos chanos (Forskall)) were analyzed, 28 from Sucol, Calamba, Laguna (Site A) and Wawa, Alabang, Metro Manila (Site B). Through the use of disc gel electrophoresis, multiple hemoglobins in complex and heterogeneous patterns ranging from 5 — 13 in site A and 5 — 14 in site B were observed, A total of 25 isohemoglobins were obtained for all samples examined. isohemoglobin 1, the fastest moving band which is uniquely expressed in site B, has an average Rf value of 0.984 while the slowest moving band, isohemoglobin 23 found in both sites, has an average Rf value of 0.138. lathodal isohemoglobins were predominantly expressed in both site: kale anodal isohemoglobins occurred in a higher frequency in as B than in site A. Statistical analysis for equality of variance of the relative mobility of isohemoglobins revealed that there is a significant difference in the variability of isohemoglobins between sites at 4,0% level of significance. The difference in variability observed can not however be correlated to the difference in oxygen content of both sites since water analysis show that both contain roughly 7.50

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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