The cytogenetic effects of wild ampalaya (Momordica charantia var abbreviata Ser) leaf decoction on human leukocytes cultured in vitro

Date

4-2002

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

ABSTRACT GATDULA, MARIA BELEN P. University of the Philippines Los Banos. March 2002. The Cytogenetic Effects of Wild Ampalaya (Momordica charantia var abbreriata Ser) Leaf Decoction on Human Leukoeytes Cultured in vitro. Undergraduate Thesis, BS Biology (Genetics). ADVISER: Dr. Merlyn S. Mendioro

Leukocytes from three normal, healthy male subjects were cultured using Peripheral Blood Technique and treated with 1%, 3%, and 5% (w/v) leaf decoction from wild ampalaya (Momordica charantia var abbreviate, Ser). One culture in each set served as the control and received only sterile saline isotonic solution. Statistical analysis showed significant decreases on mitotic indices of the treated cultures (mean mitotic indices: control = 0. 203 ; 1% = 0.167; 3% = 0.142; 5% = 0.051) and significant increases in the occurrence of condensed chromosome sets (mean frequency: control 0.093; 1% = 0.200; 3% = 0.193; 5% = 0.258). The leaf decoction of M. charantia var abbreviata Ser did not induced chromosomal aberrations in human leukocytes cultured in vitro, as shown by insignificant occurrence of gaps (control = 0.013; 1% = 0.013; 3% -- 0.027; 5% = 0.021), breaks (control = 0.013; 1% = 0.033 ; 3% = 0.040; 5% = 0.000) and loose sister chromatids (control = 0.073; 1% = 0.047; 3% = 0.127; 5% = 0.034).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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