Haploid cell culture of Saccharum officinarum L.

Date

5-1983

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Liwayway M. Engle

Abstract

Gonzalez, Eduardo Santos, College of Arts and Sciences, University of the Philippines at Los Banos, May, 1%3. Haploid Cell Culture of Saccharum offieinarum L. Adviser : Dr. Liwayway M. Engle. Anthers of S. officina rum were cultivated on a modified Murashige and Skoog liquid medium with 10-6 M 2-4 dichlorophe-noxyacetic acid and additional amino acids proline, glutamine and serine. Anthers were subjected to 4°C, 15°C and 27°C cold pre-treatment and cultivated under dark condition. Anthers pre-treated at 27°C underwent browning only at the second week of culture. Those at 15°C turned brown on the third week and those at 4°C pre-treatment, on the fourth week of culture. The latter remained slightly brown after the seventh week of culture. When dissected and exa-mined, pollen grains were observed to undergo early stages of andro-genesis. Cold pre-treatment enhanced cell to divide noire. At 4°C, 90% of the cells underwent division whereas those pre-treated at 15°C and 27°C had 70% dividing cells. Binucleate cells were high in proportion in the two latter treatments. At 4°C pre-treatment, only 5% of the grains remained viable and at 15°C there were almost 90% viable grains. Those at 27°C had 50% viable cells. Grains with actively di-viding generative nucleus and dormant or no vegetative nucleus were predominant. The pollen underwent unequal division to form the generative and vegetative nucleus which divided further to form multinucleate grains. it was, however, observed, that no apparent cell wall formation occured right after each nuclear division.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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