Isolation and identification of double stranded free nucleic acids (DS-FNA) from the water of coconut (Cocos nucifera L.)

Date

4-2006

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

To determine the type of double stranded free nucleic acid (DS-FNA) from the water coconut (Cocos nucifera L.) FNA was first from the water using ammonium acetate as precipitation salt. Approximately -0.5 ng/uL of DS-FNA was obtained using GFX PCR DNA and Gel Band Purification Kit (Amersham). Identification of the type of isolated DS-FNA was done with treatments of degradative enzymes: DNase I (Introggen), for DNA degradation: RNase A (SIgma), for RNA, polyandelic acid and polyribose phosphate degradation: and, RNase H (Introgen), specific for degradation of the RNA and DNA-RNA hybrid. Undegraded FNA were detected by using DNA Dipstick (Introgen). DNase I colorimetric test showed that the DS-FNA in coconut water has RNA and was not a double stranded DNA. The RNase H colorimetric result showed that the DS-FNA has DNA and was not a double stranded RNA. therefore, DS-FNA in the water of coconut is a DNA-RNA hybrid with the DNA strand suggested to be in the water of coconut is a DNA-RNA hybrid with the DNA strand suggested to be in degraded form as shown from the RNase A treatment results. This study set a foundation in the type of DS-FNA present in the coconut water and would be the basis for the future studies on the role of this DNA-RNA hybrid in stability and regulation in coconut endosperm.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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