In vitro cytogenetic effects of coffee (Coffea arabica L.) extracts on human leukocytes from different age groups
Date
3-2001
Degree
Bachelor of Science in Biology
College
College of Arts and Sciences (CAS)
Adviser/Committee Chair
Adelina A. Barrion
Abstract
ABSTRACT HERNANDEZ, EVA B. March 2001 University of the Philippines at Los Ratios, Laguna In Vitro Cytogenetic Effects of Coffee (Coffea arabica L.) Extracts on I Inman Leukocytes from Different Age Groups. Undergraduate Thesis B.S. Biology (Genetics)
Adviser: Adelina A Barrion, Ph D
The three age groups. 20, 37, and 55 years old male humans exhibited leukocytes' mitotic indices of 0.230, 0 140, and 0 120, respectively. When cultured leukocytes were treated with I% coffee extracts, the manifested mitotic indices were 0.160, 0.120, and 0 100, respectively Statistical analyses revealed highly significant decreasing trends in the cell proliferation with age and coffee treatments. In terms of chromosomal aberrations, frequencies of 0.030, 0.100, and 0.090 for gaps and 0.050, 0.090, and 0.090 for breaks were observed from 20, 37, and 55 years old, respectively. When the cultured leukocytes were treated with 1% coffee extracts, the observed frequencies for gaps were 0 100, 0.080, 0.060 and for breaks were 0.080, 0.060, and 0.160, respectively. Statistical analyses showed no significant increases in the chromatid gaps and breaks with age. However, with coffee treatment, a significant increase in chromatid breakage but not in gaps were observed Baseline frequencies of 0.000, 0.090, and 0.080 and coffee-induced j frequencies of 0.060, 0.070, and 0.090 for loose sister chromatids were found in the cultured leukocytes of 20, 37, and 55 years old, respectively. Statistically, age and coffee treatments showed no significant effects in loose sister chromatid pairing. The observed frequencies of chromosome condensation were 0.180, 0.040, and 0 190 from different age groups (20, 37, and 55 years old, respectively) and 0.170, 0.260, and 0.470, respectively when treated with 1% coffee extracts. Statistics of data showed significant effects of age and coffee treatment, on the formation of chromosome condensation in leukocytes. Leukocytes treated with 5% coffee exhibited shrinkage or cell death
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Hernandez, Eva B., "In vitro cytogenetic effects of coffee (Coffea arabica L.) extracts on human leukocytes from different age groups" (2001). Undergraduate Theses. 11139.
https://www.ukdr.uplb.edu.ph/etd-undergrad/11139
Document Type
Thesis
