Chromosomal study of Amaranthus species (A. viridis L., A. spinosus L., and A. dubius C. Mart. ex. Thell.)

Date

4-2003

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Sheila Marie P. Torres

Abstract

ABSTRACT ICARANGAL, CHRISELLE E. University of the Philippines Los Banos. April 2003. Chromosomal Study of Amaranthus spp. (A. viridis L., A. spinosus L. and dubius C. Mart. ex. Thell) BS Biology (Genetics).

Major Adviser: Prof. Sheila Marie P. Torres

Anther-squash technique was employed in order to determine the chromosome number and chromosomal behavior of Amaranthus viridis L., A. spinosus L., and A. dubius C. Martius ex Thell. All cells at diakinesis revealed that A. viridis and A. spinosus have 1711 (2n=34) while A. dubius has 3211 (2n=64). Chromosomal behavior in Metaphase 1 (88% for A. viridis, 92% for A. spinosus, and 94% for A. dubius), Anaphase I (84% for A. viridis, 70% for A. spinosus and 68% for A. dubius) and Telophase I (92% for A. viridis, 91% for A. spinosus and 87% for A. dubius) were highly normal but some meiotic cells exhibited chromosomal aberrations like laggards and bridges ranging from 2-13% of the observed pollen mother cells. The rate of pollen fertility of each species was also identified using 12K1. Results showed that A. viridis (96%), A. spinosus (95%) and A. dubius (93%) have high frequencies of fertile pollen cells, which reflected the normal segregation of chromosomes in the previous stages of meiosis.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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