Genetic diversity of Ralstonia solanacearum (E. F smith) Yabuuchi, Kosako, Yano, Hotta, and Nishiuchi isolated from pepper (Capsicum sp.) and tomato (Lycopersicon sp.) in Taiwan using repetitive extragenic palindromic polymerase chain reaction

Date

3-2004

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Merlyn S. Mendioro

Abstract

INIS, KATHARINE MARTINEZ. 2004. Genetic Diversity of Ralstonia solanacearum (E.F Smith) Yabuuchi, Kosako, Yano, Hotta, and Nishiuchi Isolated from Pepper (Capsicum sp) in Taiwan using Repetitive Extragenic Palindrome Polymerase Chain Reaction. University of the Philippines at Los Banos. B.S. Biology major in Genetics.

Adviser: Dr. Merlyn S. Mendioro and Dr. Jaw-fen Wang

ABSTRACT In order to study the genetic diversity in Ralstonia solanacearum (E.F Smith) Yabuuchi Kosako, Yano, Hotta, and Nishiuchi isolated from pepper and tomato, a preliminary study was conducted to compare fingerprint data of DNA extracted through boiling and Chen and Kuo's method tested by two PCR machines. The two DNA templates were amplified using Hybaid Touchdown and MJ PTC 2000 PCR machines. Differences in the banding pattern were observed using the two DNA templates. The resulting banding pattern obtained from the same strain amplified in different PCR machines proved to be different as well. Using repetitive extragenic palindromic polymerase chain reaction (REP-PCR), the genetic diversity of thirty-five strains, 29 isolated from pepper and six from tomato were determined. However, only 21 pepper strains produced confirmable results. A total of 353 bands were detected, 272 of which were polymorphic. Polymorphism ranged from 62.50-82.35%. Among the 27 strains used, only 26 banding patterns were observed where P15 and P33 exhibited the same banding pattern. The dendrogram produced by UPGMA revealed four clusters at truncation 0.60 level of dissimilarity. No well-defined clusters were observed among the pepper strains. Some tomato strains were observed to be randomly distributed with in the clusters. The dendrogram also showed that R. solanacearum pepper strains are genetically similar from 0.24 to 1.0. Results of the study showed that R. solanacearum pepper strains are genetically diverse and that R. solanacearum tomato grains are genetically similar to the pepper strains.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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