Cytology of Jasminum multiflorum Andr. and Jasminum sambac Ait. (Scrophulariales. Oleaceae)

Date

5-1992

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Teresita L. Rosario

Abstract

ABSTRACT JALANDONI, MA. LEAH MANA-AY, University of the Philippines at Los Banos, B.S. Biology, May 1992. "Cytology of Jasminum multiflorum Andr. and Jasminum sambac Ait. [Scrophulariales. Oleaceae]".

Adviser: Dr. Teresita L. Rosario

The morphology and chromosomal behavior of J. multiflorum and J. sambac were described. Pollen fertility, viability and germination on Kwack's medium were studied to assess the reason for non-fruit setting of the two species. J. multiflorum is an erect or somewhat climbing shrub, 2 to 7 meters high. There were 6 to 8 white, fragrant flowers per cyme. On the otherhand, J. sambac is a spreading or sub-scandent shrub less than 2 meters high with single white, fragrant flower per cyme. The two anthers of the flowers of both have very short filaments attached to the corolla tube. Variants with three anthers per flower have been recorded for J. multiflorum and J. sambac, Cytological observations showed normal meiosis I and II. The chromosome number of both species was confirmed to be 2n= 26. Various types of chromosomal aberrations were observed like non-congression, early disjunction and lagging chromosomes in meiosis I and II but these were of low frequency and were able to catch up with the rest of the chromosomes. Cytokinesis was observed to have taken place after division I but there was no complete separation of the cytoplasm. A high frequency of triads (76% in J. multiflorum and 67% in J. sambac) were recorded and dyads were rarely seen in both species. Pollen fertility test indicated the presence of starch but could not be considered as a sign of pollen viability. The pollen grains stained lightly and were of various sizes and shapes (tri-lobed and heart shaped pollen grains). Pollen germination test gave a conclusive evidence that both species were 100% non-viable. Pollen tube elongation was not observed from 0 to 30 hours upon incubation in Kwack's medium. The chromosome behavior at meiosis therefore, cannot be the main reason for J. multiflorum and J. sambac's incapability of setting fruits and seeds in nature.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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