Enzyme polymorphism among residents of three barangays in Los Baños, Laguna

Date

3-1985

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

ABSTRACT RESA G. JAVIER. UNIVERSITY OF THE PHILIPPINES AT LOS BANOS, MARCH, 1985. ENZYME POLYMORPHISM AMONG RESIDENTS OF THREE BARANGAYS IN LOS BANOS, LAGUNA. ADVISER : DR. RITA P. LAUDE Horizontal starch gel electrophoresis at pH 8.6 revealed enzyme polymorphism in four of the five enzyme systems investigated in blood hemolysates from 138 randomly selected persons from Barangay Anos, Mayondon and San Antonio, Los Banes, Laguna. Alcohol dehydrogenase (ADH) exhibited only one form ADH 1 with Rf value of 0.41. Lactate dehydrogenase (LDH) showed three forms LDH 1, LDIL 2 and LDH 3 with respectively, Rf values 0.39, 0.30, 0.20. The three forms were present to 66.0% of the samples while 34.0% expressed LDH 1 and LDH 3. Two -molecular forms of acid phosphatase (AcPh) were shown: AcPh 1 with Rf of 0.49 and AcPh 2 with Rf of 0.28. The banding pattern exhibited either AcPh 2 alone (58.1%) or together with AcPh 1 (41.9%). Malate dehydrogenase (NDH) was resolved into three molecular species NDH 1, NDH 2 and PDS 3 with respective mobilities of 0.49, 0.26, and 0.23. MDH3seemed to follow recessive autosomal. inheritance. It has not been linked to any genetic disorder. Glucose-6-phosphate dehydrogenase revealed two bands: G6PD 1 with Rf of 0.43 was present together with G6PD 2 with Rf of 0.26 in 7.0% of the samples and G6PD 2 alone was present in the remaining 93.0%. G6PD seemed to be controlled by an X-linked gens. It may be associated with some form of drug-induced anemia. Confirmatory tests and greater sample size are recommended to establish the mode of genetic control for each isozymes pattern.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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