Morphological, cytological and preliminary isozyme characterization of bago, Gnetum gnemon Linn. (Gnetales: Gnetaceae)

Date

10-2001

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

ABSTRACT

LABATOS, BONIFACIO JR. VIVAS. University of the Philippines Los Banos, B.S. Biology, October 2001. Morphological, Cytological and Preliminary Isozyme Characterization of Bago, Gnetum gnomon L. (Gnetales: Gnetaccae).

Adviser: Celia B. dela Villa

Four accessions of bago, Gnetum gnomon Linn. (0896-A and 13, 0238, and 0239) were characterized based on their morphology, cytology and isozyme banding pattern. Results showed that the morphological characteristics of male and female bago were almost similar except with the size of leaves and flower-like structure, which were larger in the latter. Stomates were present both at the adaxial and abaxial sides of the leaves. Bean-shaped guard cells were flanked by two to three subsidiary cells. All stages of meiosis were normal although 4.61% of Metaphase 1 cells showed laggards. There were 12 bivalents observed at Diakinesis; therefore, bago has a diploid chromosome number of 2n = 24. Mitosis was also normal. Pollen fertility was 94.43%. Phosphogluconate dehydrogenase (PGD), phosphoglucomutase (PGM) and malate dehydrogenase (MDH) were the enzyme systems used in isozyme analysis. Preliminary results showed that PGD and PGM were monomorphic whereas malate dehydrogenase was found polymorphic with three presumptive isoloci designated as MDH-1, MDH-2 and MDH-3.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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