Micromorphological variability of the Solanun nigrum complex (Solanum L. section Solanum

Date

4-2005

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Neilyn P Ona-Villa

Abstract

LACUANAN, HANNAH COLLADO. 2005.University of the Philippines Los Banos. Micromorphological variability of the Solanum nigrum Complex (Solarium section Solanum). BS Biology major in Cell Biology. Adviser: Prof. Neilyn Co-adviser: Dr. Liwayway S. Engle ABSTRACT The micromorphological variability of 15 accessions representing three species of the Solarium nigrum complex was assessed using micromorphological characters such as stomatal types, pavement cell morphology, trichome types, trichome surface morphology stomata! lengths and indices, and trichome density in 11 epidermal locations. Whole young seedlings, as well as epidermal peels from the mature plants were stained with Safranin 0, subjected to an alcohol series and xyllene, permanently mounted with entellan and observed using light microscopy. Three types of stomates, namely the anomocytic, anisocytic and tetracytic were identified in the section. Morphology of these cells were the same in all the accessions observed. Pavement cell morphology was also constant in the section. On the other hand, there were two hair classes identified in all the accessions observed: the finger hair class, which may either be glandular or eglandular, and the multicellular glandular hairs. The group showed variability in terms of the surface morphology of these trichomes with some of the accessions showing "hybrid" trichome surface morphology. These accessions are TS01879, TS01882, TS01883, tentatively identified as S. americanum from the Philippines and Vietnam, respectively, as well as TS01437 and TS02767, an S. villosum and S. nigrum from Japan and Kenya, respectively. These "hybrids" showed intermediate characters of the parental species. In addition to that, the section also showed variability in terms of the stomatal lengths and indices. Based on the trichome densities, however, minimal variability was observed in the section. The dendrogram that was constructed using trichome densities showed that the accessions coming from the Philippines, from Vietnam and the Philippines, Africa, Bangladesh, Japan, formed separate clusters.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Document Type

Thesis

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