Molecular characterization of the B-mannanase genes from coconut (Cocos nucifera L.) and rice (Oryza sativa L.)

Date

4-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Genaleen Q. Diaz

Abstract

LACHICA, GRACE C. 2009. Molecular Characterization of B-mannanase Gene from Coconut (Cocos nucifera L.) and Rice (Oryza sativa L.). University of the Philippines Los Banos. B.S. Biology Major in Genetics. Adviser: Dr. Ma. Genaleen Q. Diaz ABSTRACT The B-mannanase gene from coconut and rice was amplified, cloned, sequenced and sequence-analyzed. The primer was designed from the available sequence of the japonica rice genome, LOC_Os02g52800. Both the complementary DNA (eDNA) and genomic DNA (gDNA) of makapuno, coconut and rice were used as a template in PCR. There were no PCR products when eDNA was used. The amplified gene when the gDNA of the coconut was used was 615 by long and 1128 by when rice gDNA was used. Based on BLASTN, no 13-mannanase sequence was found to be of any similarity with the putative gene from coconut. The rice nucleotide sequence, Mar, and amino acid sequence, MAR were submitted to BLASTN and BLASTP. Results showed the high similarity of the submitted sequence with various B-mannanases from different species and a rice gene, LOC_Os02g52800. Comparing the two putative B-mannanases from rice and coconut revealed a very low similarity. The coconut 13-mannanase gene was not successfully obtained.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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