Molecular profiling of cultivated rice (Oryza sativa L.) and wild species using Sub1 markers

Date

4-2009

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Ivan Marcelo A. Duka

Abstract

LADO, JICKERSON PITOGO. 2009. Molecular Profiling of Cultivated Rice (Oryza sativa L.) and Wild Species using Sub1 Markers. University of the Philippines Los Banos. B.S. Biology Major in Cell and Molecular Biology. Adviser: Prof. Ivan Marcelo A. Duka Co-adviser: Dr. Darshan S. Brar

ABSTRACT

Molecular analysis was carried out on submergence tolerance gene, Sub], on four cultivated (IR64, BR 11, Swarna and Samba Mahsuri) varieties of rice including recurrent parents and donor, FR 13A. In this study, 32 microsatellite markers specific for chromosome 9 were utilized. SC3, a marker specific for Sub], showed similar banding pattern between donor and recurrent parents indicating that the gene of interest was transferred to these four mega varieties. Interestingly, another marker, RM316 linked to Sub1, also showed similar result to SC3. A set of 25 additional markers specific for chromosome 9 did not show any linkage drag. Four wild species of rice comprising of 26 genotypes of 0. glaberrima, 10 of 0. rufipogon, 0. longistaminata and 0. coaretata were also surveyed for the presence of Sub1 gene. It was interesting to note that only one of the 0. glaberrima lines, accession 104589 (TOG7291) had SC3 allele. To confirm that Sub] is present in 0. glaberrima TOG7291, further sequencing and analyses are recommended.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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