The effects of transposon Tn9 on ethanol production and other related characteristics of the local isolate zymonas mobilis PZ13

Date

4-1984

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Asuncion K. Raymundo

Abstract

An isolated spontaneous rifampicin resistant (rifr) mutant strain of a local isolate, Zymomonas mobilis PZ13, was used to study the effects of transposon Tn9 on alcohol production and other related characteristics of Zymomonas. A plasmid bearing transposon Tn9 (chloramphenicol resistant) was transferred via conjugation from the donor strain CsCherichia coli PGS 27 to Z. mobilis PZ13 rifr. The trans-conjugants obtain.... were chloramphenicol ana rifampicin resistant and were found to produce significantly less alcohol than the parent strain. No significant differences were observed in the flocculation and levan production of the parent strain and the transconjugants. Plasmid isolation and detection by gel electrophoresis showed that only some of the trans conjugants had plasmids. Only the strains exhibiting plasmids were also resistant to ampicillin al-though all the trans conjugant strains were chloramphenicol resistant. This indicates that only the former strains possess the plasmids originally present in the donor strain, although all of them acquired and maintained Tn9. Since decreased alcohol production was observed in all the trans-conjugants, the transposon possibly transferred from the plasmid to the bacterial chromosome and integrated in the gene of the operon for alcohol production thereby interfering with gene expression.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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