Genetic variation in natural populations of Asian honey bees, Apis cerara F. in Mt. Makiling and along Laguna De Bay areas using isozyme analysis

Date

4-1998

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

ABSTRACT Lapis, Hazel R. 1998. College of Arts and Sciences. University of the Philippines at Los Banos. Genetic Variation in Natural Populations of the Asian Honey Bee, Apis cerana F. in Mt. Makiling and Along Laguna de Bay Area Using Isozyme Analysis.

Major Adviser : Dr. Rita P. Laude

Starch gel electrophoresis of 45 nursed bees per feral colony of A. cerana F. from 3 localities in an upland area ,Mt. Makiling and lowland areas along Laguna de Bay showed polymorphism for acid phosphatase-2 (Acph-2), alkaline phophatase (Alph). esterases (Est) and malic enzyme (ME) while monomorphism for acid phophatase-1 (Acph-1). In terms of the presumptive isozymes in each locus, S (slow), M (moderate) and F (fast) were noted for Alph-2, Est-2 and ME; only S and F for Acph-2. Alph- I , Est-1 and Est-3 while only S for Acph-1. Based on the presumptive loci noted, genotype's Acph-I SS , Acph-2 SS and were observed at frequencies of 1.0 , 0.667 and 0.333 in the upland areas while 1.0 0.756 and 0.007 in the lowland areas , respectively . Likewise genotype's Alph-1 SS an FF were observed at frequencies of 0.356 and 0.644 (upland) , 0.652 and 0.348 (lowland). On the other hand, Alph-2 SS, MM and FF had genotype frequencies of 0.348, 0.229 and 0.422 respectively. Est- I SS, SF and FF genotypes were observed at frequencies o1 0.444, 0.111 and 0.444 respectively. In contrast , Est-2 SS, MM, MF, FF and SF had

genotype frequencies of 0.274, 0.222, 0.111, 0.111 and 0.281 respectively. While Est-3 SS, SF and FF had genotype frequencies of 0.659, 0.333 and 0.006. Malic enzyme's SS,SM. MM, MF, FF and SF , on the other hand, had 0.348, 0.141, 0.171, 0.237, 0.052 and 0.044 genotype frequencies respectively. Unique phenotypes observed were: Acph-2 SF in Sta Cruz, Alph-2 MM observed in San Pablo and Anos, Est-1 SF exhibited in Forestry, Est-2 MM noted in Sta Cruz and Anos, Est-2 MF in Sta Cruz, Est-3 SF noted in Sta Cruz and ME MF unique to Sta Cruz and Anos. Chi-square test for the genotype frequencies relative to the 8 presumptive loci showed a goodness-of-fit to the expected under the Hardy Weinberg equilibrium for 2 loci specifically in Acph-1 and Est-2 in the upland areas and for 2 loci namely Acph-1 and Acph-2 in the lowland areas. Localities within each area showed very high degree of genetic identity and did not vary much in terms of the types of alleles. The two areas had equal proportion of polymorphic loci (P) however greater average heterozygosity (1-1) was observed in Laguna de Bay populations. No significant difference in enzyme variability was observed between the pooled upland and lowland populations of Laguna based on P,H, average number of alleles per loci (A), genetic identity (IN), genetic distance (D) and genotypic similarity (1H).

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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