Characterization and partial purification of root phosphoenolpyruvate carboxylase (PEPC) in salt- sensitive and salt-tolerant corn (Zea mays L.) inbreds

Date

3-1997

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Evelyn Mae Tecson-Mendoza

Abstract

Laude,Teri-Marie P. University of the Philippines Los Banos. Characterization and Partial Purification of Root Phosphoenolpyruvate Carboxylase (PEPC) in Salt-sensitive and Salt-tolerant Corn (Zea mays L.) Inbreds.

Adviser: Dr. Evelyn Mae T. Mendoza

This study was conducted to characterize phosphoenolpyruvate carboxylase (PEPC) from salt-sensitive (Pi 11) and salt-tolerant (Pi 31) corn inbreds based on their optimum pH, pH and temperature stability, and Km values; and to partially purify root PEPC from Pi 31 grown in 0 mM and 25 mM NaCl. Results showed that Pi 11 root PEPC had a broad pH optimum of pH 4-8 while that of Pi 31 exhibited its optimum activity at pH 8. Pi 11 was stable over the pH range of 6-8, and temperature range of 10-30°C. Pi 31 which was stable over the pH range of 7-8 and a wider temperature range of 5-50°C. A slight difference was noted in their Km value for phosphoenolpyruvate (PEP) with PEPC from Pi 31 being greater. by 2 units. Partial purification of PEPC in Pi 31 grown at 0 mM NaCI and 25 mM NaC1 using Superdex gel column exhibited three elution peaks with molecular weights approximated to be 700,000, 160,000 and 60,000. Shifts in the first two elution peaks were noted to increase in Pi 31 grown at 25 mM NaCl. However, the third peak remained consistent for the two treatments.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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