Effects of gamma radiation on female corn earworm Helicoverpa armigera Hubner (Lepidoptera : Noctuidae)

Date

10-1998

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Pablo P. Ocampo

Co-adviser

Virginia R Ocampo

Abstract

LAUAN, MA. CLARET B. University of the Philippines, Los Banos, October 1998. Effects of Gamma Radiation on Female corn Earworm, Helicoverpa armigera Hubner (Lepitoptera Noctuidae),

Adviser: DR. PABLO P. OCAMPO

The effects of irradiating female pupae of the corn earworm(CEW), Helicoverpa armigera Hubner, with Cobalt-GO gamma rays were investigated at doses of 0 Grey (Gy), 100 Gy, 200 Gy, and 400 Gy. The ovaries of the 24-hr-old and 72-hr-old adults were obtained, and histopathologically prepared and evaluated. Among adults that emerged from irradiated pupae, test radiation dose significantly influence the relative sizes of the nurse cells and the oocytes. At 100 Gy, the cell sizes decreased 0.03 - 0.10 from the control; 200 Gy, 0.11 - 0.21; and 400 Gy, 0.19 - 0.25. Resultant adult females were sterile upon treatment of 400 Gy; no F1 offspring was produced. Histological analysis revealed structural degeneration and destruction of the ovaries of CEW under this treatment. Even at low doses, the F1 progeny of irradiated female and non-irradiated male CEWs have inherited deleterious effects. Significant reduction of the nurse cell and oocyte sizes were also detected; at 100 Gy, size difference from the control was 0.01 - 0.03, and at 200 Gy, 0.04 - 0.07.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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