Detection of mRNA coding for a putative acyl carrier protein in the water of coconut (Cocos nucifera L.) using 3' rapid amplification of cDNA ends (3' race)

Date

4-2006

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

LAZAGA, AUDRIE ANNE LEGASTO. 2006. Detection of mRNA Coding for a Putative Acyl Carrier Protein in the Water of Coconut (Cocos nucifera L.) Using 3' Rapid Amplification of cDNA Ends (3' RACE). University of the Philippines Los Banos. B. S. Biology major in Cell and Molecular Biology. Major Adviser: Dr. Rita P. Laude ABSTRACT To study the presence of mRNA in the water of coconut (Cocos nucifera L.), a protocol that would allow cDNA synthesis using the free nucleic acid (FNA) isolated as a template was optimized. To free the mRNA from the FNA, the following pre-treatments were initially done: four set-ups of FNAs were subjected to polysaccharide extraction, heat-employment, DNAse 1 treatment and Oligo(dT) extraction, respectively. There was no polysaccharide isolated in Set-up I, indicating the absence of FNA-PS complex. Set-ups 2, 3, and 4 were subjected to cDNA synthesis and amplification using 3' Rapid Amplification of cDNA Ends (3' RACE) method. Amplification products were obtained from Set-ups 3 and 4. Thus, DNAse I treatment and Oligo(dT) extraction are both effective preliminary procedures to 3' RACE with the FNA from coconut water as the starting material. Using 3' RACE, an mRNA encoding for a putative acyl carrier protein was detected. This implies that the coconut water can be used as a template for future molecular works on coconut.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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