Partial biochemical characterization of crucifer specific attractant of cabbage head caterpillar (Crocidolomia binotalis Zeller)

Date

10-2003

Degree

Bachelor of Science in Biology

Major Course

Major in Cell Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Rita P. Laude

Abstract

LAZARO, MARIANNE CHERISE CALUZA. 2003. Partial Biochemical Characterization of Crucifer Specific Attractant of cabbage head caterpillar (Crocidolomia binotalis Zeller). University of the Philippi!) Los Banos. B.S. Biology major in cell biology.

Adviser: Dr. Rita P Laude

ABSTRACT Chemicals from common cabbage (Brassica oleracea var. capitata L.) were isolated using volatile collection and microwave extraction techniques to determine whether cabbage head caterpillar (Crocidolomia binotalis Zeller) is attracted to the chemicals produced by the plant. The chemicals were separated into different organic fractions through column chromatography and the organic components were subjected to individual bioassays to test whether any of the isolated chemicals are active insect attractants. Some chemicals obtained through microwave extraction were found to attract cabbage head caterpillar while chemicals isolated through volatile collection failed to attract the insects. The microwave extracted chemical sample was further separated into its components to identify and further characterize the individual components of the said chemical attractant. However, the individually isolated chemicals did not attract the insect during bioassay. It was therefore concluded that there are certain combinations of these individual chemicals that could attract cabbage head caterpillar.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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