Further studies on the in vitro culture of mouse hepatocytes in coconut water media

Date

5-1982

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

William G. Padolina

Abstract

Dissociated mouse hepatocytes were cultured in coconut water (CW) fortified with 10% bovine serum over two observation periods of nine and five days, respectively. Using dye exclusion, spectrophotometric of the enzyme succinate dehydrogenase, and a visual examination of cellular integrity as indices of visibility, these cultures were compared with a set grown in a standard medium, Eagle's Minimum Essential Medium (MEM).

Both cultures media supported declining all populations, the rates of decrease having no significant differences. Hepatocytes cultured in CW though, showed less ability to aggregate than those cultured in MEM, and had a high incidence of bloating. Furthermore , unlike the hepatocytes cultured in MEM, they showed no significant activity of succinate dehydrogenase. It was hypothesized that these effects were caused by CW being a nutritionally and ionically imbalanced medium.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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