Meiosis in three Philippine native orchids, Dendrobium bullenianum Rchb f., Dendrobium secundum (Bl.) Lindl., and Trichoglottis geminata J.J. Smith

Date

4-2008

Degree

Bachelor of Science in Biology

College

College of Arts and Sciences (CAS)

Adviser/Committee Chair

Celia B. Dela Viña

Abstract

Meioses in Dendrobium bullenianum Rchb. f., Dendrobium secundum (BO Lindl., and Trichoglottis geminata J.J. Smith were studied using acetocarmine squash technique. Flower buds were collected from different locations and fixed in freshly made Farmer's solution between 10am and 12noon. Chromosome number, meiotic chromosome behavior and pollen fertility were determined. There were 19 II observed in all cells of the three species at diakinesis, showing a diploid chromosome number of 2n=38. In general, Meiosis I and II were normal in all the three orchid species. Laggards were observed only in Anaphase I and Metaphase II of D. bullenianum at 26.32% and 13.33% respectively, and in Metaphase I (13.33%) and Anaphase I (7.32%) of T. geminata. No laggards were observed in D. secundum. Because of high frequencies of cells with normal meiotic chromosome behavior, high pollen fertility percentages were obtained in all three species — 71.25% in D. bullenianum, 89.11% in D. secundum, and 77.97% in T. geminata.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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